In this time of dire need, Sepragen team is doing its part to solve the problem of COVID-19. We are providing equipment on an expedited basis to a company developing a vaccine for COVID- 19.
Learning Center

Learning Center

Welcome to the Sepragen Learning Center!

 

Technical Support

Documentation

QuantaSep® 100 – Complete system specifications for the QuantaSep® 100 presented in a narrative format

QuantaSep® 1000 LC – Complete system specifications for the QuantaSep® 1000 LC presented in a narrative format

WEDGE® Column – Online version of the printed User’s Manual.

Application Notes

Superflo® Column Applications – Various applications using Superflo® Columns

Superflo® Column Scaling-Up Applications – Various applications using Superflo® Columns involving scale-up

Adsorptive Radial Column Filtration (ARCF) – Improved throughput and product recovery in large scale productions on activated Aluminum Oxide and Silica.

Technical Notes

Column Packing – The simplicity and validatability of packing your Superflo® column

Scaling Up – Calculating the scale-up parameters

Automating Your Process Development – Examples of using QuantaSep® systems to speed up your process development

Gradient Formation – Breakdown of various techniques used for gradient formation in process chromatography systems.

Specifications

QuantaSep® Specifications – Detailed specifications of the QuantaSep® 100, 1000, 1000LX, 1800 and 5000 presented in a table format

Superflo® Process Scale Column – Detailed System Specifications for Superflo® Radial Process Scale Columns presented in a table format

Superflo® Lab Scale Columns – Detailed System Specifications for the Superflo® Radial Lab Prep Scale Columns presented in a table format

Superflo® Pilot Scale Column – Detailed System Specifications for Superflo® Radial Pilot Scale Steel Columns presented in a table format

How to Replace the Flow Path for The QuantaSep Adept Systems

Standard QuantaSep without pre column sensors - Trusty

QantaSep Trusty (Standard)Flow test Rate#Pumps#Inlets#Fractions#ColumnsGradientFlow Direction - Fwd / Reverse / BypassPre column Bubble Trap or Air Sensor / Auto Air EjectDual wavelength UV/ pH/ Conductivity SensorPressure rating and Pressure sensorLeak SensorPlumbing/manifolds/ fittings and piping/ tubingComputer and cGMP compliant software
Trusty 1000 - 100ml/min26 + 6103YesYesPre Column Auto Air EjectPost column6BarYesPP/PTFEYes
Trusty 100010ml/min - 1L/min26 + 6103YesYesPre Column Auto Air EjectPost column6BarYesPP/reinforced silicone rubberYes
Trusty 1000LX0 - 100ml/min, 10ml/min - 1L/min2* 2(6 + 6 )*210*23*2YesYesPre Column Auto Air EjectPost column6BarYesPP/PTFE/reinforced silicone rubberYes
Trusty 180020ml/min - 1.8L/min26 + 6103YesYesPre Column Auto Air EjectPost column3BarYesPP/reinforced silicone rubberYes
Trusty 500050ml/min - 5L/min24 + 461YesYesPre Column Auto Air EjectPost column3BarYesPP/reinforced silicone rubberYes
Trusty 10000100 ml/min - 10L/min24 + 461YesYesPre Column Auto Air EjectPost column3BarYesPP/reinforced silicone rubberYes
Trusty 300000.8L/min - 30L/min24 + 441YesYesPre Column Auto Air EjectPost column6 BarYesPPYes
Trusty 600001.2L/min - 60L/min24 + 441YesYesPre Column Auto Air EjectPost column6BarYesPPYes

Note:These systems have have Pre column detectors, Inline Buffer dilution or Precolumn Filters

All Systems have dual 280 nm and 254 nm UV detection

Quantasep software is 21 CFR Pt 11 compliant: Easy P&ID window for operation and display; manual and automated recipes; time, volume, bed volume and sensor driven step changes; event and alarm logs; chromatogram display, analysis and overlays; On-line calibration wizard; Journal; OPC-UA for connectivity; CSV

All systems are sanitary design and wetted materials are USP Grade VI and ADI free

QantaSep Buffer Blending - Trusty Elite (Standard)Flow Rate#Pumps#Inlets#Fractions#ColumnsGradientFlow Direction - Fwd / Reverse / BypassPre column Bubble Trap or Air Sensor / Auto Air EjectUV/ pH/ Conductivity SensorFlow MeterPre Column pH & conductivityBuffer/pH/Conductivity ControlPressure Rating and SensorLeak SensorPre Column FilterPlumbing/manifolds/ fittings and piping/ tubingComputer and cGMP software
Trusty Elite 5000500ml/min - 5L/min53 x 561YesYesPre Column Auto Air EjectPost column62+2Yes3BarYesYesPPYes
Trusty Elite 100001L/min - 10L/min53 x 561YesYesPre Column Auto Air EjectPost column62+2Yes3BarYesYesPPYes
Trusty Elite 300003L/min - 30L/min53 x 541YesYesPre Column Auto Air EjectPost column62+2Yes4BarYesYesPPYes
Trusty Elite 600006L/min - 60L/min53 x 541YesYesPre Column Auto Air EjectPost column62+2Yes4BarYesYesPPYes

Note: These systems have have Pre column detectors, Inline Buffer dilution or Precolumn Filters

All Systems have dual 280 nm and 254 nm UV detection

Quantasep software is 21 CFR Pt 11 compliant: Easy P&ID window for operation and display; manual and automated recipes; time, volume, bed volume and sensor driven step changes; event and alarm logs; chromatogram display, analysis and overlays; On-line calibration wizard; Journal; OPC-UA for connectivity; CSV

All systems are sanitary design and wetted materials are USP Grade VI and ADI free

QuantaSep with inline buffer dilution - Trusty Premier

QantaSep Inline Buffer Dillution - Trusty Premier (Standard)Flow Rate#Pumps#Inlets#Fractions#ColumnsGradientFlow Direction - Fwd / Reverse / BypassPre column Bubble Trap or Air Sensor / Auto Air EjectUV/ pH/ Conductivity SensorFlow MeterPre Column pH & conductivityInline Buffer dilutionPressure Rating and SensorLeak SensorPre Column FilterPlumbing/manifolds/ fittings and piping/ tubingComputer and cGMP software
Trusty Premier 5000500ml/min - 5L/min24 + 461YesYesPre Column Auto Air EjectPost column2yesYes3BarYesYesPPYes
Trusty Premier 100001L/min - 10L/min24 + 461YesYesPre Column Auto Air EjectPost column2yesYes3BarYesYesPPYes
Trusty Premier 300003L/min - 30L/min24 + 441YesYesPre Column Auto Air EjectPost column2yesYes6BarYesYesPPYes
Trusty Premier 600006L/min - 60L/min24 + 441YesYesPre Column Auto Air EjectPost column2yesYes6BarYesYesPPYes

Standard Single Use QuantaSep-Adept

QuantaSep Adept (Single Use)#Flow pathsFlow Rate ranges#Pumps#Inlets#Fractions#ColumnsFlow Direction - Fwd / Reverse / BypassPre column Bubble Trap or Air Sensor / Auto Air Ejectdual UV/ pH/ Conductivity SensorPressure rating and SensorLeak SensorTubingComputer and cGMP software
Adept 300SU20 - 100 ml/ min and 10-300 ml/min26 + 661YesYesYes3 barYesreinforced silicone rubberYes
Adept 3000SU310 - 300 ml/min, 30 - 1500ml/min & 80 - 3000 ml/min24 + 461yesYesYes3 barYesreinforced silicone rubberYes
Adept 10000SU250 - 5000 ml/ min and 100-10000 ml/min24 + 461yesYesYes3 barYesreinforced silicone rubberYes
Adept 16000SU280 - 8000 ml/ min and 160-16000 ml/min24 + 461yesYesYes3 barYesreinforced silicone rubberYes

All Systems have dual 280 nm and 254 nm UV detection

Quantasep software is 21 CFR Pt 11 compliant: Easy P&ID window for operation and display; manual and automated recipes; time, volume, bed volume and sensor driven step changes; event and alarm logs; chromatogram display, analysis and overlays; On-line calibration wizard; Journal; OPC-UA for connectivity; CSV

All systems are sanitary design and wetted materials are USP Grade VI and ADI free

Single Use QuantaSeps with inline buffer dilution- Adept Premier

Single Use QuantaSeps with inline buffer dilution- Adept Premier# Interchangeable Flow pathsFlow Rate ranges#Pumps#Inlets#Fractions#ColumnsGradientFlow Direction - Fwd / Reverse / BypassPre column Bubble Trap or Air Sensor / Auto Air EjectUV/ pH/ Conductivity SensorFlow MeterPre Column pH & conductivityInline Buffer dilutionPressure Rating and pressure sensorLeak SensorPre Column FilterDisposable tubing and manifolds and connectorsComputer and cGMP software
Adept Premier 300SU210 - 100 ml/ min and 30-300 ml/min26 + 661YesYesYesYes2YesYes (unlimited flow rate range)3 barYesyesreinforced silicone rubber/ PPYes
Adept Premier 3000SU330 - 300 ml/min, 300 - 1500ml/min & 800 - 3000 ml/min24 + 461YesYesYesYes2YesYes (unlimited flow rate range)3 barYesyesreinforced silicone rubber/ PPYes
Adept Premier 10000SU2500 - 5000 ml/ min and 1L-10L/min24 + 461YesYesYesYes2YesYes3 barYesyesreinforced silicone rubber/ PPYes
Adept Premier 16000SU2800 - 8000 ml/ min and 1.6L-16L/min24 + 461YesYesYesYes2YesYes3 barYesyesreinforced silicone rubber/ PPYes
All Systems have dual 280 nm and 254 nm UV detection

Note: These systems have have Pre column detectors, Inline Buffer dilution or Precolumn Filters

All Systems have dual 280 nm and 254 nm UV detection

Quantasep software is 21 CFR Pt 11 compliant: Easy P&ID window for operation and display; manual and automated recipes; time, volume, bed volume and sensor driven step changes; event and alarm logs; chromatogram display, analysis and overlays; On-line calibration wizard; Journal; OPC-UA for connectivity; CSV

All flowpaths are presterilized sanitary design and wetted materials are USP Grade VI and ADI free; column connectors are T/C; buffer connectors are quick connects

Single Use QuantaSeps with buffer blending - Adept Elite

Single Use QuantaSeps with buffer blending - Adept Elite#Interchangeable Flow pathsFlow Rate ranges#Pumps#Inlets#Fractions#ColumnsGradientFlow Direction - Fwd / Reverse / BypassPre column Bubble Trap or Air Sensor / Auto Air EjectFlow MeterPre Column pH & conductivityUV/ pH/ Conductivity SensorPre Column pH & conductivity controlPressure Rating and pressure sensorLeak SensorPre Column FilterProduct contact disposable tubing and manifolds and connectorsComputer and cGMP software
Adept Elite 300SU210 - 100 ml/ min and 30-300 ml/min56 + 661YesYesYes62+2YesYes3 barYesyesreinforced silicone rubber/ PPYes
Adept Elite 3000SU330 - 300 ml/min, 300 - 1500ml/min & 800 - 3000 ml/min54 + 461YesYesYes62+2YesYes3 barYesyesreinforced silicone rubber/ PPYes
Adept Elite 10000SU2500 - 5000 ml/ min and 1L-10L/min54 + 461YesYesYes62+2YesYes3 barYesyesreinforced silicone rubber/ PPYes
Adept Elite 16000SU2800 - 8000 ml/ min and 1.6L-16L/min54 + 461YesYesYes62+2YesYes3 barYesyesreinforced silicone rubber/ PPYes

Note: These systems have have Pre column detectors, Inline Buffer dilution or Precolumn Filters

All Systems have dual 280 nm and 254 nm UV detection

Quantasep software is 21 CFR Pt 11 compliant: Easy P&ID window for operation and display; manual and automated recipes; time, volume, bed volume and sensor driven step changes; event and alarm logs; chromatogram display, analysis and overlays; On-line calibration wizard; Journal; OPC-UA for connectivity; CSV

All flowpaths are presterilized sanitary design and wetted materials are USP Grade VI and ADI free; column connectors are T/C; buffer connectors are quick connects

Continuous Chromatography Capture - Fusion

Continuous Chromatography Capture - FusionFlow Rate#Pumps#Inlets#Fractions#ColumnsFlow Direction - Fwd / Reverse / BypassPre column Bubble Trap or Air Sensor / Auto Air EjectPost Column dual UV/ pH/ Conductivity SensorPressure SensorLeak SensorPre Column Filter
Fusion10 - 100ml/ min SU & 30 - 300 ml/ min22 + 43x33yes for each each columnyes for each column3 set2yesno
Fusion 300010 - 300 ml/min, 30 - 1500ml/min & 80 - 3000 ml/min22 + 43x33yes for each each columnyes for each column3 set2yesno
Fusion 1000050 - 5000 ml/ min and 100-10000 ml/min22 + 43x33yes for each each columnyes for each column3 set2yesno
Fusion 1600080 - 8000 ml/ min and 160-16000 ml/min22 + 43x33yes for each each columnyes for each column3 set2yesno

Continuous Chromatography Capture inline buffer dilution - Fusion Premier

Continuous Chromatography Capture inline buffer dilution - Fusion PremierFlow Rate#Pumps#Inlets#Fractions#ColumnsFlow Direction - Fwd / Reverse / BypassPre column Bubble Trap or Air Sensor / Auto Air EjectUV/ pH/ Conductivity SensorPre Column pH & conductivityInline Buffer dilutionPressure SensorLeak SensorPre Column Filter
Fusion Premier10 - 100ml/ min SU & 30 - 300 ml/ min22 + 43x33yes for each each columnyes for each column3 set2 setyes1yesno
Fusion Premier 300010 - 300 ml/min, 30 - 1500ml/min & 80 - 3000 ml/min22 + 43x33yes for each each columnyes for each column3 set2 setyes1yesno
Fusion Premier 1000050 - 5000 ml/ min and 100-10000 ml/min22 + 43x33yes for each each columnyes for each column3 set2 setyes1yesno
Fusion Premier 1600080 - 8000 ml/ min and 160-16000 ml/min22 + 43x33yes for each each columnyes for each column3 set2 setyes1yesno

Continuous Chromatography Capture Buffer Blending - Fusion Elite

Continuous Chromatography Capture Buffer Blending - Fusion Elite
Flow Rate#Pumps#Inlets#Fractions#ColumnsFlow Direction - Fwd / Reverse / BypassPre column Bubble Trap or Air Sensor / Auto Air EjectUV/ pH/ Conductivity SensorPre Column pH & conductivityInline Buffer BlendingPressure SensorLeak SensorPre Column Filter
Fusion Elite10 - 100ml/ min SU & 30 - 300 ml/ min55 x 33x33yes for each each columnyes for each column3 set2 setyes6yesno
Fusion Elite 300010 - 300 ml/min, 30 - 1500ml/min & 80 - 3000 ml/min52 + 43x33yes for each each columnyes for each column3 set2 setyes6yesno
Fusion Elite 1000050 - 5000 ml/ min and 100-10000 ml/min52 + 43x33yes for each each columnyes for each column3 set2 setyes6yesno
Fusion Elite 1600080 - 8000 ml/ min and 160-16000 ml/min52 + 43x33yes for each each columnyes for each column3 set2 setyes6yesno

Empty Superflo Columns

Purpose / UseNameVolumeBed Hight / DepthPressureConstructionApplications
AcrylicSSIEXAffinityDesaltingGel FiltrationReverse Phase
Lab / R&D
Superflo 5050 ml3.5 cm50 psiYesNoYesYesNoNoNo
Superflo 100100 ml3.5 cm50 psiYesYesYesYesNoNoSS only
Superflo 250250 ml3.5 cm50 psiYesYesYesYesNoNoSS only
Superflo 500500 ml3.5 cm50 psiYesYesYesYesNoNoSS only
Superflo 10001000 ml3.5 cm50 psiYesYesYesYesNoNoSS only
Superflo 15001500 ml3.5 cm50 psiYesYesYesYesNoNoSS only
Pilot
Superflo 2L2L 5 cm50 psiYesYesYesYesNoNoSS only
Superflo 3L3L 5 cm50 psiYesYesYesYesNoNoSS only
Superflo 5L5L 5 cm50 psiYesYesYesYesNoNoSS only
Superflo 7.5L7.5L 5 cm50 psiYesYesYesYesNoNoSS only
Superflo 10L10L 5 cm50 psiYesYesYesYesNoNoSS only
Process
Superflo 10L10 L10 cm50 psiYesYesYesYesYesNoSS only
Superflo 20L20 L10 cm50 psiYesYesYesYesYesNoSS only
Superflo 30L30 L10 cm50 psiYesYesYesYesYesNoSS only
Superflo 40L40 L10 cm50 psiYesYesYesYesYesNoSS only
Superflo 50L50 L10 cm50 psiYesYesYesYesYesNoSS only
Superflo 60L60 L10 cm50 psiYesYesYesYesYesNoSS only
Superflo 70L70 L10 cm50 psiNoYesYesYesYesNoSS only
Superflo 80L80 L10 cm50 psiNoYesYesYesYesNoSS only
Superflo 90L90 L10 cm50 psiNoYesYesYesYesNoSS only
Superflo 100L100 L10 cm50 psiNoYesYesYesYesNoSS only
Industrial
Superflo 150L150 L15 cm50 psiNoYesYesYesYesNoSS only
Superflo 350L350 L15 cm50 psiNoYesYesYesYesNoSS only

Note: Do not use use Acrylic columns with Alcohol

Prepacked Superflo columns

Purpose / UseNameVolumeBed Hight / DepthPressureMaterial of ConstructionApplications
Scale up from Lab to Production scale Radial flow columns of 10L to 100 L sizeAcrylicSSIEXAffinityDesaltingGel FiltrationReverse Phase
Wedge 55 ml10 cm
Wedge 1010 ml10 cm50 psiYesNoYesYesYesNoNo
Wedge 2020 ml10 cm50 psiYesYesYesYesYesNoSS only
Wedge 5050 ml10 cm50 psiYesYesYesYesYesNoSS only
Wedge 100100 ml10 cm50 psiYesYesYesYesYesNoSS only
Wedge 250250 ml10 cm50 psiYesYesYesYesYesNoSS only
Wedge 500500 ml10 cm50 psiYesYesYesYesYesNoSS only
Wedge 10001000 ml10 cm50 psiYesYesYesYesYesNoSS only

Note: Custom Pack with your choice of media

Empty Wedge Columns - 10 cm bed depth

Purpose / UseNameVolumeBed Hight / DepthPressureMaterial of ConstructionApplications
Scale up from Lab to Production scale Radial flow columns of 10L to 100 L sizeAcrylicSSIEXAffinityDesaltingGel FiltrationReverse Phase
Wedge 55 ml10 cm
Wedge 1010 ml10 cm50 psiYesNoYesYesYesNoNo
Wedge 2020 ml10 cm50 psiYesYesYesYesYesNoSS only
Wedge 5050 ml10 cm50 psiYesYesYesYesYesNoSS only
Wedge 100100 ml10 cm50 psiYesYesYesYesYesNoSS only
Wedge 250250 ml10 cm50 psiYesYesYesYesYesNoSS only
Wedge 500500 ml10 cm50 psiYesYesYesYesYesNoSS only
Wedge 10001000 ml10 cm50 psiYesYesYesYesYesNoSS only

Prepacked Wedge columns - 10 cm bed depth

Purpose / UseNameVolumeBed Hight / DepthPressureSepraprep SSepraprep QMaterial of ConstructionApplications
Scale up from Lab to Production scale Radial flow columns of 10L to 100 L sizeAcrylicIEXAffinityDesalting
Wedge 55 ml10 cm50 psiyesyesYesYesYesYes
Wedge 1010 ml10 cm50 psiyesyesYesYesYesYes
Wedge 2020 ml10 cm50 psiyesyesYesYesYesYes
Wedge 5050 ml10 cm50 psiyesyesYesYesYesYes
Wedge 100100 ml10 cm50 psiyesyesYesYesYesYes
Wedge 250250 ml10 cm50 psiYesYesYesYesYesYes
Wedge 500500 ml10 cm50 psiYesYesYesYesYesYes
Wedge 10001000 ml10 cm50 psiYesYesYesYesYesYes
Note: Pack with your choice of media

Empty Wedge Columns - 15 cm bed depth

Purpose / UseNameVolumeBed Hight / DepthPressureMaterial of ConstructionApplications
Scale up from Lab to columns over 150L Radial Flow columnsAcrylicSSIEXAffinityDesaltingGel FiltrationReverse Phase
Highflo 1010 ml15 cm
Highflo 2020 ml15 cm50 psiYesNoYesYesYesNoNo
Highflo 5050 ml15 cm50 psiYesYesYesYesYesNoSS only
Highflo 100100 ml15 cm50 psiYesYesYesYesYesNoSS only
Highflo 250250 ml15 cm50 psiYesYesYesYesYesNoSS only
Highflo 500500 ml15 cm50 psiYesYesYesYesYesNoSS only
Highflo 10001000 ml15 cm50 psiYesYesYesYesYesNoSS only
Highflo 15001500 ml15 cm50 psiYesYesYesYesYesNoSS only

Prepacked 15 cm. Wedge columns

Scale up from Lab to columns over 150L Radial Flow columnsAcrylicIEXAffinityDesalting
Highflo 1010 ml15 cm50 psiyesyesYesYesYesYes
Highflo 2020 ml15 cm50 psiyesyesYesYesYesYes
Highflo 5050 ml15 cm50 psiyesyesYesYesYesYes
Highflo 100100 ml15 cm50 psiyesyesYesYesYesYes
Highflo 250250 ml15 cm50 psiyesyesYesYesYesYes
Highflo 500500 ml15 cm50 psiYesYesYesYesYesYes
Highflo 10001000 ml15 cm50 psiYesYesYesYesYesYes
Highflo 15001500 ml15 cm50 psiYesYesYesYesYesYes
Note: Pack with your choice of media

How to Chose Radial flow columns

Bed Height / DepthResearchProcess DevelopmentSmall Scale MfgMedium scale Mfg.Large scale mfgVery Large scale mfg
Superflo Lab Columns3.5 cmYYY
Superflo Pilot Columns5 cmYY
Superflo Process Columns10 cmYY
Superflo Large Process Columns15 cmYY
Superflo PP Columns3.5 cmYYY
Wedge Columns10 cmYYY
Wedge PP Columns10 cmYYY
Wedge HighRes Columns15 cmYYY
Wedge HighRes PP Columns15 cmYYY
Wedge HighRes Columns should be used when scaling up to Superflo Large Process Columns

Note: These columns are ideal for on off separations like IEX, Affinity and Reverse Phase and ideal for Cell culture based products like Recombinant Proteins, MAB'S, Gene Therapy, Plasma derived products, and various products derived from Whey

Superflo Lab columns should be used for R&D or small scale manufacturing of MAB's for Diagnostics or Gene Theraphy

Superflo Pilot columns can be used for production of Larger volumns of MAB for diagnostic purposes or Gene Therapy products

Superflo Process Columns should be used for production of MAB's etc for therapeutics purposes

Superflo Large Process Columns should be used for Large volumn products like Plasma or Dairy process

Wedge columns should be used when scaling up to Superflo Process columns

Wedge HighRes Columns should be used when scaling up to Superflo Large Process Columns