In this time of dire need, Sepragen team is doing its part to solve the problem of COVID-19. We are providing equipment on an expedited basis to a company developing a vaccine for COVID- 19.
Knowledge Center
Processes for Health-Enhancing Human Nutritionals
In the creation of bio-pharmaceutical drugs, cells are genetically modified to produce some compound shown by research to have a desired effect in a treatment program. First there’s the genetic engineering to discover a compound and then how to modify a cell to produce the compound. Essentially the cell becomes a chemical factory secreting a certain compound. The cells are then grown in a manner similar to how beer is produced in vats called “bioreactors”.

These cells typically produce very big macro molecules (ranging in molecular weight from 50,000 to well over 100,000), most are proteins, some are peptides. This differs from traditional pharmaceuticals which are generally made from much smaller molecules.
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Enabling Biological Drugs Scale Up & Production
Sepragen’s products or tools are used to manufacture over 12 FDA approved drugs including Kogenate®, Alphanate®, Neupogen®, and GammaGrand®* along with a multitude of other drugs currently in clinical trials.
Sepragen’s customers range from manufacturers of recombinant biological protein and peptides, vaccines manufacturers, derivates, genetic therapy vectors, monoclonal antibody producers for diagnostic and therapeutic use and enzyme producers. Our international list of customers on four continents includes a combination of big pharma and more entrepreneurial biotech companies: Amgen, Bayer, Grifols, Chiron, Cohesion, Burroughs Wellcome, Eli Lilly, Baxter, Asahi Glass, Aventis, Merck, Human Genome Sciences, CSL, Smithkline Beecham, Chugai, Schering Plough, IDEC, Hoffman La Roche, Roche Molecular Systems, Genencor, Berlex, Dade, Yoshitomi.

In one instance, over 40,000 liters of human plasma is being treated every 6 hours on 2,800 liters of Sepragen columns to produce tens of kilograms of an injectable protein drug.
Biologic Drug Manufacturing
Sepragen has pioneered the three patented processes for the isolation of high value nutritionals on a commercial scale. The Sepralac® Process enables the isolation of high value whey protein isolate, alphalactalbumin, lactoferrin and CGMP for use in infant formulae, performance foods and other functional foods. Commercial plants capable of processing over 100,000 liters of whey per day have been commissioned. Sepradebitt® process to debitter and de-acify citrus juice and the Sepraflavone® Process to isolate the natural “phyto” estrogen of Isoflavone from soy are currently in commercial evaluations with potential licenses.
  • Sepralac® Process: The isolation of certain proteins from whey for use in infant formulae, performance foods and other functional foods
  • Sepradebitt® Process: debitter and de-acify citrus juice
  • Sepraflavone® Process: isolate the natural “phyto” estrogen of Isoflavone from soy

 

Documentation
Application Notes
Technical Notes
QantaSep Buffer Blending - Trusty Elite (Standard)Flow Rate#Pumps#Inlets#Fractions#ColumnsGradientFlow Direction - Fwd / Reverse / BypassPre column Bubble Trap or Air Sensor / Auto Air EjectUV/ pH/ Conductivity SensorFlow MeterPre Column pH & conductivityBuffer/pH/Conductivity ControlPressure Rating and SensorLeak SensorPre Column FilterPlumbing/manifolds/ fittings and piping/ tubingComputer and cGMP software
Trusty Elite 5000500ml/min - 5L/min53 x 561YesYesPre Column Auto Air EjectPost column62+2Yes3BarYesYesPPYes
Trusty Elite 100001L/min - 10L/min53 x 561YesYesPre Column Auto Air EjectPost column62+2Yes3BarYesYesPPYes
Trusty Elite 300003L/min - 30L/min53 x 541YesYesPre Column Auto Air EjectPost column62+2Yes4BarYesYesPPYes
Trusty Elite 600006L/min - 60L/min53 x 541YesYesPre Column Auto Air EjectPost column62+2Yes4BarYesYesPPYes

Standard Single Use QuantaSep-Adept

QuantaSep Adept (Single Use)#Flow pathsFlow Rate ranges#Pumps#Inlets#Fractions#ColumnsFlow Direction - Fwd / Reverse / BypassPre column Bubble Trap or Air Sensor / Auto Air Ejectdual UV/ pH/ Conductivity SensorPressure rating and SensorLeak SensorTubingComputer and cGMP software
Adept 300SU20 - 100 ml/ min and 10-300 ml/min26 + 661YesYesYes3 barYesreinforced silicone rubberYes
Adept 3000SU310 - 300 ml/min, 30 - 1500ml/min & 80 - 3000 ml/min24 + 461yesYesYes3 barYesreinforced silicone rubberYes
Adept 10000SU250 - 5000 ml/ min and 100-10000 ml/min24 + 461yesYesYes3 barYesreinforced silicone rubberYes
Adept 16000SU280 - 8000 ml/ min and 160-16000 ml/min24 + 461yesYesYes3 barYesreinforced silicone rubberYes

Single Use QuantaSeps with inline buffer dilution- Adept Premier

Single Use QuantaSeps with inline buffer dilution- Adept Premier# Interchangeable Flow pathsFlow Rate ranges#Pumps#Inlets#Fractions#ColumnsGradientFlow Direction - Fwd / Reverse / BypassPre column Bubble Trap or Air Sensor / Auto Air EjectUV/ pH/ Conductivity SensorFlow MeterPre Column pH & conductivityInline Buffer dilutionPressure Rating and pressure sensorLeak SensorPre Column FilterDisposable tubing and manifolds and connectorsComputer and cGMP software
Adept Premier 300SU210 - 100 ml/ min and 30-300 ml/min26 + 661YesYesYesYes2YesYes (unlimited flow rate range)3 barYesyesreinforced silicone rubber/ PPYes
Adept Premier 3000SU330 - 300 ml/min, 300 - 1500ml/min & 800 - 3000 ml/min24 + 461YesYesYesYes2YesYes (unlimited flow rate range)3 barYesyesreinforced silicone rubber/ PPYes
Adept Premier 10000SU2500 - 5000 ml/ min and 1L-10L/min24 + 461YesYesYesYes2YesYes3 barYesyesreinforced silicone rubber/ PPYes
Adept Premier 16000SU2800 - 8000 ml/ min and 1.6L-16L/min24 + 461YesYesYesYes2YesYes3 barYesyesreinforced silicone rubber/ PPYes
All Systems have dual 280 nm and 254 nm UV detection

Single Use QuantaSeps with buffer blending - Adept Elite

Single Use QuantaSeps with buffer blending - Adept Elite#Interchangeable Flow pathsFlow Rate ranges#Pumps#Inlets#Fractions#ColumnsGradientFlow Direction - Fwd / Reverse / BypassPre column Bubble Trap or Air Sensor / Auto Air EjectFlow MeterPre Column pH & conductivityUV/ pH/ Conductivity SensorPre Column pH & conductivity controlPressure Rating and pressure sensorLeak SensorPre Column FilterProduct contact disposable tubing and manifolds and connectorsComputer and cGMP software
Adept Elite 300SU210 - 100 ml/ min and 30-300 ml/min56 + 661YesYesYes62+2YesYes3 barYesyesreinforced silicone rubber/ PPYes
Adept Elite 3000SU330 - 300 ml/min, 300 - 1500ml/min & 800 - 3000 ml/min54 + 461YesYesYes62+2YesYes3 barYesyesreinforced silicone rubber/ PPYes
Adept Elite 10000SU2500 - 5000 ml/ min and 1L-10L/min54 + 461YesYesYes62+2YesYes3 barYesyesreinforced silicone rubber/ PPYes
Adept Elite 16000SU2800 - 8000 ml/ min and 1.6L-16L/min54 + 461YesYesYes62+2YesYes3 barYesyesreinforced silicone rubber/ PPYes

Continuous Chromatography Capture - Fusion

Continuous Chromatography Capture - FusionFlow Rate#Pumps#Inlets#Fractions#ColumnsFlow Direction - Fwd / Reverse / BypassPre column Bubble Trap or Air Sensor / Auto Air EjectPost Column dual UV/ pH/ Conductivity SensorPressure SensorLeak SensorPre Column Filter
Fusion10 - 100ml/ min SU & 30 - 300 ml/ min22 + 43x33yes for each each columnyes for each column3 set2yesno
Fusion 300010 - 300 ml/min, 30 - 1500ml/min & 80 - 3000 ml/min22 + 43x33yes for each each columnyes for each column3 set2yesno
Fusion 1000050 - 5000 ml/ min and 100-10000 ml/min22 + 43x33yes for each each columnyes for each column3 set2yesno
Fusion 1600080 - 8000 ml/ min and 160-16000 ml/min22 + 43x33yes for each each columnyes for each column3 set2yesno

Continuous Chromatography Capture inline buffer dilution - Fusion Premier

Continuous Chromatography Capture inline buffer dilution - Fusion PremierFlow Rate#Pumps#Inlets#Fractions#ColumnsFlow Direction - Fwd / Reverse / BypassPre column Bubble Trap or Air Sensor / Auto Air EjectUV/ pH/ Conductivity SensorPre Column pH & conductivityInline Buffer dilutionPressure SensorLeak SensorPre Column Filter
Fusion Premier10 - 100ml/ min SU & 30 - 300 ml/ min22 + 43x33yes for each each columnyes for each column3 set2 setyes1yesno
Fusion Premier 300010 - 300 ml/min, 30 - 1500ml/min & 80 - 3000 ml/min22 + 43x33yes for each each columnyes for each column3 set2 setyes1yesno
Fusion Premier 1000050 - 5000 ml/ min and 100-10000 ml/min22 + 43x33yes for each each columnyes for each column3 set2 setyes1yesno
Fusion Premier 1600080 - 8000 ml/ min and 160-16000 ml/min22 + 43x33yes for each each columnyes for each column3 set2 setyes1yesno
Specifications
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