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Overview and Advantages

Sepragen’s Sepraflo Resins are designed for process scale separation of biological molecules. They can be run at very high flow rates, typically up to 50% higher than commonly used resins from other suppliers. They have large nominal 300A pore size that facilitates rapid mass transfer of large macromolecules and provides for high static and dynamic binding capacity. They are cleanable with 1N sodium hydroxide.

Sepraflo resins consist of macroporous beads with nominal 50 micron size. They are rigid and can withstand pressures in excess of 500 psi. They can be supplied by Sepragen in batches of 100 liters on up. The resins are made of a hydrophilic copolymer of glycidyl methacrylate which is used predominantly in making contact lenses. The Sepraflo resins are priced at a fraction of the price of competitive resins.

The resins are available in both a strong cation exchange or sulpho propyl chemistry and a strong anion exchange quaternary amino ethyl chemistry.

Application: Antibody Separation on Sepraflo S resin

The graph below shows an antibody separation on a Sepraflo cation exchange resin. Elution is done with a salt gradient.

Flow rate vs. Pressure Performance comparison

The data below shows a comparison of the flow rates that can be achieves with various resins at varying pressures. Sepraflo resins consistently show higher flow rates than all other resins.
Ion Exchange Binding Capacity of Sepraflo Resins

The data below shows the binding capacity of Sepraflo resins when compared with other commercially available resins. The resins show comparable binding with a model protein BSA. Binding capacities will vary for different proteins based on their charge, size and molecular weight, PI, tertiary structure, and the pH and ionic strength of the solution and the competitive binding effect of other counterions in solution.

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